testosterone t levels  (Monobind)

Journal: Frontiers in endocrinology

Article Title: Type 1 diabetes impairs the activity of rat testicular somatic and germ cells through NRF2/NLRP3 pathway-mediated oxidative stress.

doi: 10.3389/fendo.2024.1399256

Figure Lengend Snippet: FIGURE 3 Analysis of LC activity in control and T1D rat testis. (A) Testicular Testosterone levels and (B) WB analysis of StAR, 3b-HSD, CYP17A1, and CYP19A1 protein levels. (C–F) Histograms showing StAR, 3b-HSD, CYP17A1, and CYP19A1 relative protein levels quantified using ImageJ and normalized to b- actin. (G) Testicular StAR (red) and 3b-HSD (green) immunolocalization. The slides were counterstained with DAPI-fluorescent nuclear staining (blue). The images were captured at ×20 (scale bars = 20 µm) magnification and ×40 (scale bars = 10 µm) for the insets. Asterisks, LC. (H, I) Histogram showing the quantification of StAR and 3b-HSD fluorescence signal intensity, respectively. (J) WB analysis of INSL3 and RXFP2 protein levels. (K, L) Histograms showing INSL3 and RXFP2 relative protein levels quantified using ImageJ and normalized to b-actin. All values are expressed as means ± SEM from five animals in each group. *p < 0.05; **p < 0.01.

Article Snippet: Testicular testosterone level measurement Testosterone (T) levels were determined in the testis of both groups using a commercial kit (#582701; Cayman Chemical Company, Michigan, MI, USA) and using a previously published protocol for the sample’s treatment (45).

Techniques: Activity Assay, Control, Staining

Journal: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Article Title: Expression and functional analysis of Fushi Tarazu transcription factor 1 (FTZ-F1) in the regulation of steroid hormones during the gonad development of Fujian Oyster, Crassostrea angulata.

doi: 10.1016/j.cbpa.2024.111668

Figure Lengend Snippet: Fig. 4. FTZ-F1 expression and steroid hormone levels in the gonads of C. angulata across different time points (0 days, 3 days, 7 days and 15 days) after RNAi. (A) FTZ-F1 expression levels in both control and RNAi groups (*0.01 < P < 0.05; ***P < 0.001; N = 9). (B) Estradiol (E2) content in both control and RNAi groups (*0.01 < P < 0.05; **0.001 < P < 0.01; N = 9). (C) Testosterone (T) content in both the control and RNAi groups (N = 9). Because the gonads of the RNAi group exhibited poor development, male and female individuals were indistinguishable. Therefore, we used all individuals after RNAi as the experimental group for comparisons.

Article Snippet: Estradiol (E2) and testosterone (T) levels were determined using a commercial ELISA kit (Cayman Chemical Company, USA), and their concentrations were determined using standard curves provided by the manufacturer (Cayman Chemical Company, USA).

Techniques: Expressing, Control